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While effective for protein engineering and metabolic pathway optimization, CCCambird is currently limited by the availability of cell-free extracts capable of supporting complex post-translational modifications (e.g., glycosylation). Additionally, the cost of linear DNA synthesis at scale remains higher than traditional plasmid propagation.

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: The app was first released in November 2018 and received its last update on November 25, 2018. cccambird 48h renewed work